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An en route inspection should be comprehensive and include multiple checks that ensure both vehicle safety and cargo security. Checking for tire overheating is crucial as tires can heat up from under-inflation or overloading, which could lead to blowouts or reduced control over the vehicle. Additionally, ensuring that cargo is properly secured is equally important, as unsecured cargo can shift during transit, causing imbalances that may lead to accidents or increases in stopping distances.

By combining these checks as part of an en route inspection, a driver can help maintain the safety of the vehicle and the load, ensuring compliance with safety regulations and preventing potential accidents. Therefore, a thorough inspection should involve monitoring for both tire conditions and the security of the cargo. This comprehensive approach is what makes the combined option the most correct choice for a proper en route inspection.